Un protoplasto es una célula de planta, bacteria u hongo que ha perdido total o parcialmente su pared celular, para lo cual se usan. Cultivo y fusion de protoplastos []. Loyola Vargas, V.M. Rosell, C.H. (ed.) Villalobos Arambula, V.M. (ed.) Access the full text: NOT AVAILABLE. Lookup the . El poder seleccionar eficientemente las plantas híbridas producidas a partir de fusión de protoplastos es fundamental. Se han logrado muchos avances en el.

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Heterokaryons can not be identified because protoplasts of the parents were of the same type and shape. Nineteen somatic hybrid plants were d from fusion experiments using PEG in an effort to combine elite traits from both parents. The production of somatic hybrid plants with a chromosome number deviating from the expected is well lrotoplastos in many fusion combinations where potato protoplasts have been used as one or both fusion parents Austin et al.

When colonies developed into microcalli, the alginate beads were depolimerized. Your institution must subscribe to JoVE’s Biology section to access this content.

Plant Cell Rep 3: The mixture was incubated for thirty minutes and then protoplasts were collected by centrifugation at rpm during five minutes. Cubra la placa con una tapa.

Tissue culture procedures to produce somatic hybrids and the application of molecular biological tools for their analysis are well established. Production of somatic hybrids between frost – tolerant Solanum commersonii and Solanum tuberosum: An unexpected error occurred.

Transferencia de los fusionn resuspendidos en un pocillo de la placa de protoplwstos. Transfer of resistance to potato leaf roll virus from Solanum brevidens into Solanum tuberosum by somatic fusion.


Fusion de Protoplastos by Arely Gutierrez on Prezi

The ce treatment was carried out overnight with gentle agitation. Protoplast fusion, culture and regeneration. None of the plants had a similar banding pattern in relation to each one of the parents.

After seven days, the culture medium was diluted 1: Many wild Solanum species are regarded as important sources for disease resistance and tolerance to many abiotic stresses Hawkes, but their use in potato breeding is limited due to poor crossability and sterility of interspecific hybrids.

Tissue culture response in various wild and cultivated Solanum germplasm accessions for exploitation in potato breeding. Skip to content Biology. Somatic hybridization fueion the genus Solanum: Somatoclonal variation in potato: These plants were multiplied in glass culture tubes for proto;lastos analysis.

Protoplasts fusion from S. Aggregations of more than two protoplasts were also observed. Somatic hybrids were confirmed morphologically under greenhouse conditions, when intermediate characteristics between parent lines, such as shoot coloration, leaf shape and pubescence were observed. Somatic hybrids between potato Solanum tuberosum and wild species Solanum fuison exhibit a recombination in the plastome. The wild species S. In this paper we describe the fusion of leaf protoplasts of S.

To select hybrids, regeneration of in vitro plants was subjected to RAPD analysis.

Invierta suavemente el tubo varias veces. The same primer amplified four specific bands of, and bp in S. Identification of somatic hybrids is a requisite for the effective exploitation of the protoplasts fusion in potato improvement.

These samples were obtained from rooted plants maintained in a greenhouse. Protoplast fusion between haploid gametic and diploid somatic cells has been achieved which clearly demonstrate that fusiln is independent of cell type.

Fusion de protoplastos pdf

The authors would like to thank the International Potato Center for financial support and to Martha Williams de Castro who revised the English version of this article. The osmolality of this medium was fixed at mOsm. Root tips were stained by lacto-propionic orcein. Then, at fifty days, all culture media were replaced by a mOsm V-KM medium.


Both 6carboxyfluorescein and the kanamycin resistance harboured by the plasmid have been transferred from liposomes to protoplasts of phycomyces dw the fusion technique. Protoplast fusion, culture and regeneration Prior to fusion, protoplasts from S.

Please sign in or create an account. These bands also appeared in the fusion regenerants Figs.

Cultivo y fusion de protoplastos

A subscription to J o VE is required to view this article. The RAPD analysis was carried out for all regenerated plants derived from calli of the combination S. RAPD and chromosomal analysis of the hybrids are also presented. Con una cuchilla de afeitar nueva, retire la nervadura central de las hojas unifolioladas y luego corte los restos en 0. Bigg boss 9 yesterday episode on dailymotion Public choice economics theory books Garmin sd card download Holiday joy english subtitles download Ms office project download free Jane film download vf youdownload divergente Kingdom episode 2 vf sword art download season 14 dub full episodes Ricoh aficio mf driver Dofus book sram 1 2 amys Miguel ayuso pdf writer.

Protoplasts of both types are mixed in an equal proportion attaining a density of 5 x 10 4 to 2 x 10 5 protoplasts per ml.

A revised medium for rapid growth bioassays with tobacco tissue culture.