BACTERIAS DEGRADADORAS DE HIDROCARBUROS AISLADAS EN SUELOS CONTAMINADOS DE PETROLEO Aislar cepas bacterianas y. Aislamiento de bacterias potencialmente degradadoras de petróleo en hábitats de ecosistemas costeros en la Bahía de Cartagena, Colombia. Download Citation on ResearchGate | SELECCIÓN DE BACTERIAS CON CAPACIDAD DEGRADADORA DE HIDROCARBUROS Estudio y selección de bacterias aerobias degradadoras de hidrocarburos del petróleo aisladas de costas.

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Taxonomy of aerobic marine eubacteria. The cultures were discarded after 15 days. Several authors have pointed out that short chain aliphatic hydrocarbons usually volatilize during the first hours after a spill. Some authors degradavoras some species of this genus into Alteromonas instead [17].

Three replicates were seeded per strain.

Each of the images shown in the figure contains the profiles of the abiotic control and that of the strain under examination, for a number of different carbon chain lengths.

These microorganisms can be petroldo not only in soil and water samples, but in clinical specimens, occasionally.

Figure 3 shows the chromatographic profile of the saturated hydrocarbon fraction, in a signal intensity pico-amperes versus time chart for strains F9S, F10S1 and F1FLC. Anaerobic microorganisms, however, are less versatile regarding their growth substrate and often display increased sensitivity toward heavy metals, hence playing a smaller role in biodegradation [2]. Taxonomic scheme for the identification of marine bacteria.

Culturing these strains in the presence of hydrocarbons Mesa 30 crude favored the expression of enzyme systems involved in their degradation and the preferential isolation of hydrocarbon-tolerant clones. Measures taken after the Exxon Valdez spill included the addition of nutrients in the form of fertilizers Inipol EAP 22 and Customblerwhich increased the rate of oil removal badterias three-fold [6].

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Venosa A, Zhu X. Online J Biol Sci. In addition, it must be noted that synthesis of the enzyme complexes required to degrade the heavier fractions is not induced until lighter fractions have been exhausted, following the principle of maximum cellular economy gacterias regulation.

Publications reporting the presence of vacterias genus in hydrocarbon-contaminated ecosystems and describing its hydrocarbon-degrading abilities have appeared in the literature from the early nineties [17], although its numbers have increased as of late [19, 24, 25, 31, 32].


However, and despite the higher propensity of n-alkanes for oxidation [35, 49], no differences in biodegradation percentages were detected between saturated hydrocarbons, asphaltenes and monocyclic aromatic hydrocarbons after 45 days. Bacterias marinas con capacidad bzcterias aisladas de moluscos bivalvos en cultivos. Published reports indicate that they can also degrade phenanthrene in soils [38] as well as anthracene, phenol [41] and methyl methylbromide in marine environments [40]. Pure cultures of these strains were further used in crude oil biodegradability assays.

Introduction to the Archaea by JuanCho Rios on Prezi

Biodegradation of petroleum hydrocarbons by psychrotrophic Pseudomonas strains possessing both alkane alk and naphthalene nah catabolic pathways. Universidad de Oriente; Biochemical characterization of the strains comprised tests for the fermentation of glucose, lactose, sucrose and mannitol, as well as for the production of indole, gas and segradadoras sulfide.

Bacterial metabolism is readjusted as culture ages and less complex substrates saturated linear alkanes and low molecular weight aromatic compounds are exhausted, shifting towards the synthesis of enzyme complexes geared towards the degradation of more complex molecules.

At the end, 18 strains representing each unique combination of these parameters were chosen to be subjected to the selection process. In both protocols the marine bacteria isolation plates were periodically examined after 24 h of growth under a stereoscope, streaking onto separate plates all the colonies appearing during the seven day incubation period. The strains Alcaligenes sp.

Description of toluene inhibition of methyl bromide biodegradation in seawater and isolation of a marine toluene oxidizer that degrades methyl bromide.

Viewed as a whole, the latter possess the necessary genetic information petrolfo produce all the enzymes required to completely degrade complex hydrocarbon mixtures in damaged areas [4, 5].

BACTERIAS DEGRADADORAS DE HIDROCARBUROS by Mirelly Katherine Diaz Gamarra on Prezi

Strain F10S1 degraded Comparative study of five polycyclic aromatic hydrocarbon degrading bacterial strains isolated from contaminated soils. This phenomenon was more pronounced for strains F10S and F9S, which did not exhibit statistically significant differences when compared in this regard, but did so when compared to strain F1FLC. F9S and Bacillus sp. Degrsdadoras latter, in turn, are more susceptible to biodegradation than resins and asphaltenes [47, bacrerias.


These compounds did not mineralize completely, accumulating instead as intermediary metabolites consisting of linear chains and aromatic compounds. Enrichment, isolation, and phylogenetic identification of polycyclic aromatic hydrocarbon-degrading bacteria from Elizabeth River sediments. Phenotypic characterization of the strains Degradadiras strains were characterized phenotypically using previously described morphological, physiological and biochemical tests [], using previously defined criteria to describe culture deegradadoras [13].

We were unable to find previous mentions degradadorxs the literature of the presence of these microorganisms in hydrocarbon-contaminated environments. Biodegradation of Polycyclic Aromatic Hydrocarbons. F10S1 and Bacillus sp.

Characterization of the hydrocarbon degradation capacity of the selected strains The ability to degrade hydrocarbons from crude oil Mesa 30 crude was determined after 45 days of static culture.

It has been shown that strain WW1 of Alcaligenes denitrificans can degrade four-ring polyaromatic hydrocarbons [30]. Each chart depicts both the profile of the strain under examination and that of the standard sample. Another two strains belonged to the genera Acinetobacter and Marinomonasrespectively.

All spectra were processed using the Omnic v5. Microbial diversity in a hydrocarbon- and chlorinated-solvent-contaminated aquifer undergoing intrinsic bioremediation. The present study has demonstrated that the degradadpras strains selected are able to use hydrocarbons as sole carbon source when grown in pure cultures.

Molecular characterization of a toluene-degrading methanogenic consortium. Spill Sci technol Bull. These authors attributed such a phenomenon to adaptations of these microorganisms to the chronic contamination of their original habitats. The spectra in figure 4 reveal the presence of aromatic compounds in the crudes treated with bacterial strains. In general, biodegradation is expected to be more extensive in aliphatic hydrocarbons, which are far more amenable to this process than their aromatic counterparts [35, 47, 48].

Effects of rhamnolipid biosurfactants on removal petrolek phenanthrene from soil. Biodegradation of crude oil pettoleo marine shorelines and freshwater wetlands.